By Chirag Mandavia

e-book Date: February 22, 2013 | ISBN-10: 3659348147 | ISBN-13: 978-3659348143
In order to check AT2-mediated sign transduction mechanisms, major ways have been used: (1) the ligand-binding houses of mutant AT2 receptors have been characterised compared to wild-type after iteration of some degree mutation utilizing site-directed mutagenesis, and (2) interacting protein companions have been analyzed utilizing yeast library screening, co-immunoprecipitation and Western Blot reports. We came across that the power of Angiotensin II (Ang II) receptor sort AT2 Phe308Ala residue mutant within the seventh TMD to bind peptidic ligands was once enormously decreased, utilizing Xenopus oocytes expression method. this deposit occupies a place within the AT2 analogous to the Tyr292 within the AT1 crucial for agonistic activation of that receptor. Screening of a 17-day mouse embryo cDNA library with the Angiotensin II receptor AT2 because the bait in yeast two-hybrid assay pointed out sodium-hydrogen exchanger NHE6 because the binding associate with ninety eight% series homology. additional reviews printed that after human cell-line MCF-7 was once transfected with the AT2, the AT2 co-immunoprecipitated with the NHE6 within the presence of Ang II, implying that Ang II-mediated activation is required for the interplay among AT2 and NHE6.
Show extra
Show less

Show description

Read or Download Characterization of the Angiotensin receptor AT2 by various studies PDF

Best medical research books

Principles and Practice of Clinical Trial Medicine

Scientific trials are an enormous a part of drugs and healthcare at the present time, determining which remedies we use to regard sufferers. someone inquisitive about healthcare this day needs to recognize the fundamentals of working and analyzing medical trial information. Written in an easy-to-understand kind through authors who've enormous services and adventure in either academia and undefined, ideas and perform of medical Trial drugs covers all the fundamentals of medical trials, from criminal and moral matters to statistical data, to sufferer recruitment and reporting effects.

La médecine sans médecin ? Le numérique au service du malade (Le Débat) (French Edition)

L’évolution accélérée des applied sciences bouleverse l. a. médecine et notre système sanitaire. Elle porte à ses dernières conséquences le changement que le stéthoscope de Laennec avait jadis engagé en forgeant un software qui démultiplie le pouvoir de l’observation. Nous assistons pour de bon à l’émergence de ce que man Vallancien suggest d’appeler une "média-médecine", une médecine médiatisée par le recours aux capacités de l’ordinateur, que l’on retrouve de l. a. génétique à los angeles robotique chirurgicale, en passant par los angeles télémédecine et les communautés de malades.

Managing the Laboratory Animal Facility, Third Edition

Compliment for the former Editions"The writer brings in administration knowledge from the realm outdoor laboratory animal medication and veterinary medication. for this reason, there's a wealthy mix of the adventure of a pro expert and the theoretical framework utilized by faculties of administration …. i like to recommend this booklet to managers and laboratory animal experts at any degree in their careers.

Additional info for Characterization of the Angiotensin receptor AT2 by various studies

Example text

Download PDF sample

Rated 4.81 of 5 – based on 22 votes